1. Falsely increased results may be seen in patients on high doses of biotin (i.e. > 5mg/day). Samples should only be collected from these patients at least 8 hours following the last biotin administration so as to avoid potential interference.
2. The analytical sensitivity of the Estradiol assay is 5 pg/mL and the functional sensitivity is 25 pg/mL. The functional sensitivity is defined as the lowest concentration that can be measured with a CV of ≦ 20%. Therefore, for values lower than 25 pg/mL, the reproducibility may be poor. Please review low results together with clinical findings.
Source: Roche Elecsys Estradiol III reagent package insert
Follicular phase