| 1. The results of samples should be used in conjunction with clinical evaluation and other diagnostic procedures, such as isolation techniques. |
| 2. Lack of significant rise in antibody level dose not exclude the possibility of infection. A correct interpretation of serologic data depends on the proper timing of specimen collection in relation to rash onset. Since IgM antibody peaks approximately 10 days after rash onset and declines rapidly becomes undetectable at 30 to 60 days. |
| 3. The results of a single-specimen antibody determination should not be used to aid in the diagnosis of recent infection. Paired samples (acute and convalescent) should be collected and tested concurrently to look for seroconversion or a significant rise in antibody level. |
| 4. Results in IgG detection in neonates must be interpreted with caution, since maternal IgG is transferred passively from the mother to the foetus before birth. IgM assays are generally more useful indicators of infection in children below 6 months of age. |
| 5. Enzyme immunoassay tests to detect viral antibodies has several limitations. Therefore, if clinically indicated, confirmation by other methodologies or other tests are recommended. |
|
| Source: Vircell Measles VirClia IgM Monotest package insert. 06/2014. |